Nutrient medium preparation for Spirulina culture

 To prepare a nutrient medium for *Spirulina*, the concentrations vary significantly depending on whether you are following a rigorous laboratory protocol (like Zarrouk's) or a simplified method for small-scale production.

Below are the quantities required for 1 Liter of water

 1. Zarrouk’s Medium (Laboratory Standard)

This is the scientific gold standard for optimized growth. It provides a balanced suite of macro- and micronutrients.

| Component | Amount (per Liter) |

| **Sodium Bicarbonate (NaHCO_3)** | 16.80 g |

| **Sodium Nitrate (NaNO_3)** | 2.50 g |

| **Dipotassium Phosphate (K_2HPO_4)** | 0.50 g |

| **Potassium Sulfate (K_2SO_4)** | 1.00 g |

| **Sodium Chloride (NaCl)** | 1.00 g |

| **Magnesium Sulfate (MgSO_4 \cdot 7H_2O)** | 0.20 g |

| **Calcium Chloride (CaCl_2 \cdot 2H_2O)** | 0.04 g |

| **Iron Sulfate (FeSO_4 \cdot 7H_2O)** | 0.01 g |

*Note: In professional settings, trace elements (boron, manganese, zinc, copper, molybdenum) are added in minute quantities using a concentrated stock solution.

 2. Simplified Protocol (Small-Scale/Domestic)

If you are managing a smaller unit and want to avoid complex lab-grade trace elements, this "simplified" recipe is often cited for reliable home or small-batch cultivation.

| Component | Amount (per Liter) |

| **Sodium Bicarbonate** | 16.0 g |

| **Sodium Nitrate** | 2.0 g |

| **Dipotassium Phosphate** | 0.5 g |

| **Potassium Sulfate** | 1.0 g |

| **Sodium Chloride** | 2.0 g |

| **Iron Sulfate** | 0.1 g (100 mg) |

 Critical Preparation Tips

 Dissolution Strategy: Always dissolve your **Sodium Bicarbonate** first. It acts as the primary buffer. If you try to dissolve the other minerals in acidic water, the pH may fluctuate unexpectedly.

 The Iron Factor: Iron is often the limiting factor for growth. It must be in a bioavailable form. If you are not using EDTA (a chelating agent), ensure your iron source is very finely ground and thoroughly mixed, or it may precipitate out of the solution at the high pH required for *Spirulina*.

 Water Source:  As mentioned previously, ensure your water is strictly free of chlorine. Using deionized water is preferred if you are preparing your own concentrated stocks to ensure you are not adding unknown minerals from tap or well water that could interfere with the delicate mineral balance. You can also use RO water which is pre sterilized with UV filter.

Grow spirulina at home

 Spirulina Pro: Grow Your Own Superfood at Home

Did you know you can grow one of the world’s healthiest foods right in your own home? With "Spirulina Pro", we help you set up and manage your very own 50-litre Spirulina tank in your balcony . It is simple, affordable, and highly rewarding.

Our total consultation fee is ₹5,000, but you don't have to pay it all at once. We offer a flexible installment plan so you can pay in stages as you progress with your setup.

What You Get with Spirulina Pro:

Guaranteed Spirulina Plant setup: We provide a 100% assurance in setting up working 50 litre spirulina Plant at your home. 

No Tension (Culture Crash Protection): We know you might worry about your culture dying. With our program, if your culture ever crashes, we will provide you with fresh Spirulina culture again for free. You can grow with complete peace of mind.

Regular Harvests: Learn how to harvest 10 grams of fresh, pure Spirulina every 5 days.

Easy Setup: We guide you through the entire process—from selecting the right equipment to setting up your tank. 

Nutrient Secrets: We teach you how to mix the necessary nutrients so your Spirulina grows fast and stays healthy.

Step-by-Step Harvesting: You will learn exactly how to harvest, wash, and store your Spirulina so it stays fresh for your daily use.

Lifetime Skill: Once you learn these techniques, you can grow your own Spirulina forever. It’s a one-time investment for a lifetime of health.

Support & Guidance: We don't just set it up and leave; we are here to guide you whenever you have a doubt.

Why Choose This?

Pure & Safe: You know exactly what goes into your food. No chemicals, no middleman.

Best Quality: Fresh Spirulina is much more nutritious than the dried powder you buy in stores.

Save Money: Stop spending thousands on store-bought supplements. Grow your own

!

Spirulina Culture received but seems to be spoiled.

 

Spirulina culture bottle picture sent by one of our customers after 10 days of shipment.

This is one of the problems during shipping of Spirulina culture. Due to non availability of light, temperature above 35° centigrade the culture changes its colour.

 Especially during summer hot temperature may Spirulina during transit. We at Ropecrafts biotech use special thermal Packing to protect from temperature with integrated LED system.

High temperatures pose a challenge to culture. In our lab experiments we observed that spirulina can rejuvenate again after complete crash due to high temperatures or complete absence of light. This is a survival behaviour which is observed in Spirulina. Sometimes we need to be patient and wait to see that happen. Usually it takes 5 to 20 days. 

We also provide free culture in case the culture crashes with in 20 days.

In some situations we do provide complete refund. We believe that the customer satisfaction is important. Customer should be protected.

  If the Spirulina culture is received in above state, a simple solution. Transfer the culture into a clean glass jar. Close the jar with lid and place in diffused light for 10 days. The culture will restore to its healthy condition. Do not touch the culture with any objects as it may introduce other microbes. 

 Shipping Spirulina in couriers have some limitations. 

Courier companies does not allow shipping of liquid material due to risk of spillage.

Courier companies do not use temperature controlled logistics for normal packages.

We have to use special shipping to deliver the product in good shape. Sometimes it becomes too expensive for us when we transport in temperature controlled logistics.

Note:- when Spirulina is in quantity of 1 to 5 litre, it should maintained  with care. Especially it should not be kept in direct light

When the culture quantity is above 50 l, the culture becomes strong and  can with stand direct sunlight, temperature fluctuations.

Spirulina home system 45 l

 

 

We designed 45 liters capacity Spirulina tank system equipped with water pump, Temperature control system . Ready to harvest 5 grams of Spirulina every day, that is sufficient for family consumption.  The system has cover lid which keeps away dust and debri. Pure spirulina is ready to harvest every day morning. We add nutrients weekly once. This is a brilliant innovation to grow own Spirulina at home. This is completely organic, Pure spirulina.